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very abundant (++++, i.e. over 60% of the cells). Immunocytochemistry GM-CSF-stimulated and unstimulated SaOS-2 cells were plated and grown on glass coverslips and fixed after 1, 7 and 14 days of culture with 3% formaldehyde in PBS for 3 min at room tempe

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quently re-established in culture.In addition,anoth-er advantage of our model is that early stages of cardiomyogenesis,including the initial formation of cell-cell contacts,can be studied under well-defined in vitroconditions,e.g.,with respect to RA conce

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We also attempted to apply this histogram-based quantification approach to SR stained sections however, this failed (Figure 5 B,D). The histograms of the SR staining in Figure 5D show a big overlap between the background and the whole heart histogram in t

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